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Книга ISBN13 9783846521922 - Evaluation of antiviral and cytotoxic activity (Waqas Ahmad,Muhammad Ashraf and Aqeel Javeed) в магазинах, библиотеках и электронных библиотеках с он-лайн чтением

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Название: Evaluation of antiviral and cytotoxic activity
The antiviral activity of plants Glyceriza glabra (roots), Phyllanthus amblicus (Fruit), Eugenia jamolana (Leaves), and Moringa oleifera (Leaves) were evaluated against Infectious bursal disease virus (IBDv) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus. Four dilutions of each extracts viz 100, 50, 25 and 12.5?g/ml were made. Vero cells were infected by identified infectious bursal disease for antiviral assay. Dilutions of these extracts were applied in triplicate manner on Vero cells (infected by IBDv), that are confluent in 96 well cell culture plates. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells by the same dilutions as used in antiviral assay and incubated the both 96 well cell culture plates for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol–2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Endpoint of these assays was measured...
Авторы: Waqas Ahmad,Muhammad Ashraf and Aqeel Javeed
Издательство: LAP Lambert Academic Publishing
Год: 2011
Местонахождение: OZON.ru
ISBN: 9783846521922


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10 января 2015 года: Запуск базы ISBN10 и ISBN13

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